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Old 06-15-2017, 01:59 PM   #1
imsharmanitin
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Location: cambridge,UK

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Default is this a technical replicate or biological replicate

i know that biological replicate is one taken from different organism/sample while technical replicate is one from same organism/sample.

However, I am not able to understand that a cell line treated with a drug three times separately to generate RNA-Seq data will be considered as biological replicate or technical replicate or a biological replicate but not in a strict sense. Also, will this be applicable for control sample?

Thanks in advance
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Old 06-15-2017, 02:58 PM   #2
nucacidhunter
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I think it would be biological replicates and the same goes for control which should be treated with solvent without the drug. I imagine that there will be other treatments such as exposure time and dose as well in such experiments.
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