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Old 08-20-2017, 10:34 AM   #1
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Default tophat-fusion result

Can I use the accepted_hits.bam of "tophat2 --fusion-search" to calculate the gene expression level?

Usually I just use tophat to map reads to genome, then calculate the gene expression level by the accepted_hits.bam. But when I need to rsearch the fusion transcript, I use the "tophat2 --fusion-search", then I got an accepted_hits.bam, could I use this bam file to calculate the gene expression level? I don't want to map the reads again using tophat2 without --fusion-search.

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accepted_hits.bam, fusion-search, tophat

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