SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
minimum depth of coverage for transfrag extension apadr007 Bioinformatics 6 05-15-2012 06:23 AM
New to RNA-Seq: Help obtaining sequencing summary needed. ccard28 Bioinformatics 12 05-14-2012 12:44 AM
SOLiD Small RNA - Minimum % miRNA in library DrDTonge Sample Prep / Library Generation 0 06-20-2011 02:55 AM
What is the minimum coverage needed? Annettet Illumina/Solexa 0 03-22-2011 02:41 AM
Minimum Coverage for ChIP-Seq & RNA-Seq canis Illumina/Solexa 7 07-17-2009 01:34 AM

Reply
 
Thread Tools
Old 02-22-2012, 05:09 PM   #1
hechanner
Junior Member
 
Location: Shanghai, China

Join Date: Dec 2011
Posts: 1
Question Minimum coverage needed of de novo small RNA sequencing

Hello,

I am a novice member with some novice questions.
I have data about small RNA profile of an avian species without reference genome. I get less than 1M reads per sample after filtration. My questions are: 1, how many reads per sample should be enough? 2, for analysis, should I map them to an related species' genome?
Also, I am looking for publications about de novo in small RNA.

Thanks a lot in advance for your help!
hechanner is offline   Reply With Quote
Reply

Tags
coverage, small rna

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 01:03 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO