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Old 08-20-2013, 12:42 AM   #1
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Location: Moscow, Russia

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Default Library preparation for MiSeq

I have a question library preparation for MiSeq. I want to sequence the mix of libraries, which length ranges from 300 to 700 bp. I know that sequenator preferably works with shorter sequences. So the question is: In what ratio do I have to mix the libraries of different lengths (ex 300, 500 and 700 bp) so that they have a uniform coverage.

Last edited by Mizraelson; 08-20-2013 at 02:25 AM.
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Old 08-20-2013, 08:01 AM   #2
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Unfortunately, I think this would be trial and error. I would just go ahead and mix them in equimolar amounts.
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coverage, different lenghts, miseq

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