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Old 11-02-2015, 12:32 AM   #1
Lynhuizi
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Location: Singapore

Join Date: Nov 2015
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Default Run error - no clustering

Hi,
I ran a MiSeq run last week and the run was aborted due to clustering failure.
I am not sure whether it is due to the library preparation, reagents or the machine. My run is a 2x250 cycle run and I loaded 8pm of the library in with 8% Phix spike-in.

I have worked with the first 2 panels of primers without any problems and added in Panels 3 to 6. Below is the number of primers I have for each panel.

- Panel 1 – 12 forward, 12 reverse
- Panel 2 – 14 forward, 12 reverse (same reverse as Panel 1)
- Panel 3 – 68 forward, 26 reverse
- Panel 4 – 8 forward, 4 reverse
- Panel 5 – 14 forward, 6 reverse
- Panel 6 – 14 forward, 30 reverse

Would appreciate any comments/help on this! Thank you!
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Old 11-02-2015, 01:10 AM   #2
GenoMax
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Default

Your best bet is to contact Illumina tech support. They can look at your instrument remotely and diagnose the failure. Illumina will replace the kit if they determine that the failure was due to instrument/reagents (you will need a maintenance contract for this but most people do have one).
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