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Old 11-28-2016, 07:14 AM   #1
BhariD
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Default How to identify differentially expressed genes within a single sample group?

What are the reasonable ways to identify differentially expressed genes within a Single sample group consisting of 5 replicates with RNA-seq data? There is no second or other group to compare.
Appreciate your suggestions. Thank you.
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Old 11-29-2016, 01:06 AM   #2
wdecoster
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Differentially expressed versus what?
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Old 11-30-2016, 08:15 PM   #3
BhariD
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With differentially expressed genes, I meant to say genes that are over-expressed or under-expressed within a single experimental group consisting of more than one replicates. There is no second group or 'normal' group to compare the experimental group. For example, how to identify genes over- or under-expressed in tumor samples of a specific cancer type in the absence of 'normal' samples or any other possible secondary experimental group.
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Old 12-01-2016, 03:18 AM   #4
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But "differentially expressed" implies you compare group A with group B to find differences between the groups.
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