Dear all
I need to convert GFF3 files to RefFlat; or to find how to generate RefFlat files. The reason for my question is that I need to run Picard, module CollectRnaSeqMetrics, that requires RefFlat files.
I am working on Arabidopsis Thaliana, that is not in UCSC database. This means that I cannot use UCSC RefFlat files but I have to generate them.
UCSC provides a program that seems to cope with my need: gff3ToGenePred (link: http://hgdownload.cse.ucsc.edu/admin/exe/linux.x86_64/gff3ToGenePred). In this case the program does not work with the available GFF3 for my genome, TAIR10 (this link for original GFF3 Tair10: ftp://ftp.arabidopsis.org/home/tair/...GFF3_genes.gff and ftp://ftp.arabidopsis.org/home/tair/...ransposons.gff) because of GFF3 errors: GFF3 files seem not standard GFF3.
For this reason I am also trying to find a GFF3 validator but I only found online validators that do no work.
Can anyone help me on creating a RefFlat file for genomes that are not in UCSC database?
Thank you
Andrea
I need to convert GFF3 files to RefFlat; or to find how to generate RefFlat files. The reason for my question is that I need to run Picard, module CollectRnaSeqMetrics, that requires RefFlat files.
I am working on Arabidopsis Thaliana, that is not in UCSC database. This means that I cannot use UCSC RefFlat files but I have to generate them.
UCSC provides a program that seems to cope with my need: gff3ToGenePred (link: http://hgdownload.cse.ucsc.edu/admin/exe/linux.x86_64/gff3ToGenePred). In this case the program does not work with the available GFF3 for my genome, TAIR10 (this link for original GFF3 Tair10: ftp://ftp.arabidopsis.org/home/tair/...GFF3_genes.gff and ftp://ftp.arabidopsis.org/home/tair/...ransposons.gff) because of GFF3 errors: GFF3 files seem not standard GFF3.
For this reason I am also trying to find a GFF3 validator but I only found online validators that do no work.
Can anyone help me on creating a RefFlat file for genomes that are not in UCSC database?
Thank you
Andrea
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