Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
Total RNA quality (Bioanalyzer trace attached) peromhc Sample Prep / Library Generation 26 10-12-2015 03:36 PM
Extra peaks in a bioanalyzer trace for library made from ePCR amplicon dtm2451 Sample Prep / Library Generation 15 06-09-2014 06:47 AM
Large MW seen in mRNA bioanalyzer trace ts1127 Sample Prep / Library Generation 1 07-03-2013 06:25 AM
Ideas on Bioanalyzer trace for ChIP-seq library? orlatron Sample Prep / Library Generation 0 05-14-2012 10:02 PM
Ladder-like Bioanalyzer trace on TruSeq RNA libraries egunth Sample Prep / Library Generation 2 12-01-2011 11:53 AM

Thread Tools
Old 07-11-2014, 12:33 PM   #1
Location: New Jersey

Join Date: Jun 2013
Posts: 22
Default Strange RNA Bioanalyzer trace, any thoughts?

We have only just started using the Bioanalyzer RNA 6000 Nano chips, and I haven't seen a good trace yet. Today's was very strange....

There is a main peak at ~40 seconds that blows the rest of the peaks away (one was over 1000 FU). The software is calling it 18S, but there isn't a corresponding 28S peak.

I've attached a PDF of the four traces.

I loaded 1 ul of total RNA, with concentrations of 7.2 to 31.6 ng/ul as measured by Qubit. These samples have been in -80 since May (2 months), made on 5/8/14 and thawed once on 5/9/14 to make TruSeq RNA libraries (which worked beautifully). Any thoughts on that huge peak? Thank you for your insight!
sweetph3 is offline   Reply With Quote
Old 07-14-2014, 12:03 PM   #2
MU Core
Location: Columbia, Missouri

Join Date: Apr 2008
Posts: 57

The profile is reminiscent of insect RNA that has the characteristic of the 28S breaking in to two subunits when heated. This is a phenomenon observed for many years. The attached articles demonstrates discusses this in honeybee RNA.
Attached Files
File Type: pdf Insect RNA BioAnalyzer Profile.pdf (1.41 MB, 24 views)
MU Core is offline   Reply With Quote
Old 07-14-2014, 06:36 PM   #3
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
Posts: 1,234

MU Core comments are correct. This topic has also been discussed here:
nucacidhunter is offline   Reply With Quote

rna bioanalyzer

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 03:52 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO