Hello everyone,
I'm new in this forum and just joined it today. I noticed that this forum is awesome and full of experts.
I started to prepare ampliseq library recently, my amplicons are about 400bp. I run 30 different samples every time. For some reasons, some samples have high library yield, some have low yield. It is not because of samples DNA since I usually get high yield for some of these low yield samples when I repeat them. At the beginning, I thought I overdried the AMPure beads. Then, I changed to dry my beads 5 mins as the protocol suggests for the first purification after ligation. I dry 2-5 mins for the post library amplification. I still got similar results with some library prep has higher yield, some has very low yield. I wonder if anyone has the sample problem and if anyone knows the reasons.
Thank you all very much for your reply in advance.
I'm new in this forum and just joined it today. I noticed that this forum is awesome and full of experts.
I started to prepare ampliseq library recently, my amplicons are about 400bp. I run 30 different samples every time. For some reasons, some samples have high library yield, some have low yield. It is not because of samples DNA since I usually get high yield for some of these low yield samples when I repeat them. At the beginning, I thought I overdried the AMPure beads. Then, I changed to dry my beads 5 mins as the protocol suggests for the first purification after ligation. I dry 2-5 mins for the post library amplification. I still got similar results with some library prep has higher yield, some has very low yield. I wonder if anyone has the sample problem and if anyone knows the reasons.
Thank you all very much for your reply in advance.
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