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Old 01-21-2021, 02:18 AM   #1
Mo Micro 89
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Location: New york

Join Date: Jan 2021
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Default Visualization of Transposon insertions

Hi everyone,

I have my transposon mutant library, and I want to visualize the mapped transposon insertions to the reference genome via DNAplotter (Artemis).

I need the plot to be like the attached picture from the literature (where the red lines represent the transposon insertions).

I could not do the same in Artemis because non of the output files from BioTradis pipeline are in EMBL format. I can only visualize BAM and insertion plot as shown in (My_entry). Therefore, could anyone help or suggest how can I convert the files that mapped to the reference genome (e.g., BAM, fq.gz) to EMBL in order to visualize the insertions in DNAplotter.

Thanks in advance,
Attached Images
File Type: jpg literature.jpg (44.5 KB, 0 views)
File Type: png My_Entry.png (75.8 KB, 0 views)
File Type: png my_plot.png (87.7 KB, 0 views)
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