NExtflex adapters for Truseq

rnaquestions · 01-10-2019, 04:29 PM

I am preparing RNA libraries using the TruSeq Total RNA kit from Illumina and I am trying to use NextFlex adapters in lieu of the truseq ones. I am stuck on the correct concentration to use and Illumina tells me the molarity of their adapters is proprietary. Does anyone know the correct concentration of their adapters, I don't have any on hand to test.

luc · 01-10-2019, 08:59 PM

Check the concentration of the Nextflex RNA-seq adapters on their web page; I have not memorized it but it will work.

rnaquestions · 01-11-2019, 06:21 AM

Thanks Luc, I know the concentration of the nextflex adapters and I know they will work. I am looking for the concentration of the illumina adapters

luc · 01-11-2019, 02:46 PM

Nextflex adapters are available for DNA applications and for RNA-seq applications. The latter are merely dilutions of the former, but Bioo provides the concentration info somewhere.
If you have the diluted Nextflex adapters (for RNA-seq) use the same adapter volume as in the TruSeq RNA kit.

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01-10-2019, 04:29 PM	#1
rnaquestions Junior Member Location: maryland Join Date: Jan 2019 Posts: 2	NExtflex adapters for Truseq I am preparing RNA libraries using the TruSeq Total RNA kit from Illumina and I am trying to use NextFlex adapters in lieu of the truseq ones. I am stuck on the correct concentration to use and Illumina tells me the molarity of their adapters is proprietary. Does anyone know the correct concentration of their adapters, I don't have any on hand to test.

01-10-2019, 08:59 PM	#2
luc Senior Member Location: US Join Date: Dec 2010 Posts: 453	Check the concentration of the Nextflex RNA-seq adapters on their web page; I have not memorized it but it will work.

01-11-2019, 06:21 AM	#3
rnaquestions Junior Member Location: maryland Join Date: Jan 2019 Posts: 2	Thanks Luc, I know the concentration of the nextflex adapters and I know they will work. I am looking for the concentration of the illumina adapters

01-11-2019, 02:46 PM	#4
luc Senior Member Location: US Join Date: Dec 2010 Posts: 453	Nextflex adapters are available for DNA applications and for RNA-seq applications. The latter are merely dilutions of the former, but Bioo provides the concentration info somewhere. If you have the diluted Nextflex adapters (for RNA-seq) use the same adapter volume as in the TruSeq RNA kit.