I'm looking for a parser to compile basic stats from the various html/xml/text output files from NextSeq runs. Looking for a text file to archive with fastq files that includes sample name, sample ID, indexes, total clusters per sample not per lane since NextSeq lanes are not independent. Thanks.
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
Take a look at MultiQC if you want to look at many runs.
Following is true for local bcl2fastq analysis. I am not familiar with BaseSpace but I suppose a similar structure can be found there as well.
Otherwise the index.html file found in (FCID/Unaligned/Reports/html) directory has all the stats or if you prefer a standalone file then (FCID/Unaligned/Reports/html/FC_BARCODE/all/all/all/laneBarcode.html).
JSON format results are in (FCID/Unaligned/Stats/Json.stats).Last edited by GenoMax; 03-05-2018, 11:22 AM.
-
Thanks GenoMax. We are running our NextSeq standalone.
The html file displays the metrics per lane per sample...I'd like to have the information for each sample combined into one row, e.g. Lane 1 Sample A, Lane 2 Sample A, Lane 3 Sample A, Lane 4 Sample A -> Sample A.
If you know of an html to csv or json to csv script I could handle this in Excel.
Comment
-
While there appear to be many (?) online tools following may be safer.
1. Download Atom programmers editor here.
2. Find "Settings" tab after installing Atom and click on +Install.
3. Search for a package called "json-converter" and install it.
4. Download and open Stats.json file in Atom.
5. Use Packages menu drop down, find "Json Converter" and select "Json to csv".
6. Write the converted data out to file and then do what you need to in Excel.
Comment
-
Originally posted by lac302 View PostI'm looking for a parser to compile basic stats from the various html/xml/text output files from NextSeq runs. Looking for a text file to archive with fastq files that includes sample name, sample ID, indexes, total clusters per sample not per lane since NextSeq lanes are not independent. Thanks.
I have attached a perl script I use for this purpose, however when dealing with NextSeq run data the stats are still divided by lane. The script reads the DemultiplexingStats.xml and ConversionStats.xml files within the Stats/ directory created by bcl2fastq2. It requires Perl Modules Getopt::Long and XML::LibXML. It has one mandatory input, the path to the Stats/ directory and one optional argument for parsing data from single end runs.
Code:# parseBcl2FastqStatsXml.pl [-s] -i <path>/Stats/ > output.txt The argument for -i must end in /Stats/ -s is optional for single read runs
Attached FilesLast edited by kmcarr; 03-06-2018, 09:06 AM.
Comment
-
Originally posted by jgarces View Post@kmcarr, thanks for that useful parser! One quick question: is there any way to sum each line instead of showing them separately?
Thanks!
Comment
-
Thanks for you reply, @kmcarr. Our sequencing were performed in a different lab and we haven't the BCL and this kind of run information... but at the end, with your parser and a bit of R, I was able to extract the information I wanted.
Thanks again!
Comment
Latest Articles
Collapse
-
by seqadmin
The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...-
Channel: Articles
05-06-2024, 07:48 AM -
-
by seqadmin
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
Channel: Articles
04-22-2024, 07:01 AM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, 05-10-2024, 06:35 AM
|
0 responses
19 views
0 likes
|
Last Post
by seqadmin
05-10-2024, 06:35 AM
|
||
Started by seqadmin, 05-09-2024, 02:46 PM
|
0 responses
21 views
0 likes
|
Last Post
by seqadmin
05-09-2024, 02:46 PM
|
||
Started by seqadmin, 05-07-2024, 06:57 AM
|
0 responses
20 views
0 likes
|
Last Post
by seqadmin
05-07-2024, 06:57 AM
|
||
Started by seqadmin, 05-06-2024, 07:17 AM
|
0 responses
21 views
0 likes
|
Last Post
by seqadmin
05-06-2024, 07:17 AM
|
Comment