I recently tried to annotate two assembled metagenome samples derived from the same sample type (both samples come from the same type of material) using blastp and the metagene gene predictor tool (via WebMGA). I get a similar number of ORFs predictions for both samples. However after I input these ORF predictions into blastp (same settings for both samples) the number of blastp outputs for one sample were much higher than for the other sample. Why would I get more blastp hits for samples with a similar number of ORFs predicted?
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...-
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