Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Haplotype caller 3.2 versions not calling a true variant

    Hi! I have just run the 3.2.2 and 3.2.0 versions on the same bam file and I noticed that it is not calling a true variant, while the earlier version 3.1.1 calls it. Everything in the command is identical aside from GATK haplotype caller version. Any ideas why this variant is not called and how to address that? (it's the middle variant chr10:43022236)
    Thanks for all the ideas!

    ##GATKCommandLine=<ID=HaplotypeCaller,Version=3.1-1-g07a4bf8,Date="Mon Aug 25 15:45:14 EDT
    10 42823862 . C T 125.77 . AC=1;AF=0.500;AN=2;BaseQRankSum=-1.568;ClippingRankSum=-0.633;DP=11;FS=8.751;MLEAC=1;MLEAF=0.500;MQ=57.87;MQ0=0;MQRankSum=-1.036;QD=11.43;ReadPosRankSum=0.000 GT:ADP:GQ:PL 0/1:4,6:10:99:154,0,121
    10 43022236 . A C 119.77 . AC=1;AF=0.500;AN=2;BaseQRankSum=1.368;ClippingRankSum=0.421;DP=8;FS=0.000;MLEAC=1;MLEAF=0.500;MQ=60.00;MQ0=0;MQRankSum=0.421;QD=14.97;ReadPosRankSum=-0.421 GT:ADP:GQ:PL 0/1:4,4:8:99:148,0,129
    10 43159396 . G A 21.77 LowQual AC=2;AF=1.00;AN=2;DP=2;FS=0.000;MLEAC=2;MLEAF=1.00;MQ=60.00;MQ0=0;QD=10.88 GT:ADP:GQ:PL 1/1:0,2:2:6:49,6,0


    ##GATKCommandLine=<ID=HaplotypeCaller,Version=3.2-0-g289df4b,Date="Mon Aug 25 15:46:43 EDT
    10 42823862 . C T 118.77 . AC=1;AF=0.500;AN=2;BaseQRankSum=-1.568;ClippingRankSum=-1.036;DP=11;FS=8.751;MLEAC=1;MLEAF=0.500;MQ=57.87;MQ0=0;MQRankSum=0.000;QD=10.80;ReadPosRankSum=0.365 GT:ADP:GQ:PL 0/1:4,6:10:99:147,0,124
    10 43159396 . G A 21.77 LowQual AC=2;AF=1.00;AN=2;DP=2;FS=0.000;MLEAC=2;MLEAF=1.00;MQ=60.00;MQ0=0;QD=10.88 GT:ADP:GQ:PL 1/1:0,2:2:6:49,6,0


    ##GATKCommandLine=<ID=HaplotypeCaller,Version=3.2-2-gec30cee,Date="Mon Aug 25 13:43:53 EDT
    10 42823862 . C T 118.77 . AC=1;AF=0.500;AN=2;BaseQRankSum=-1.568;ClippingRankSum=-0.365;DP=11;FS=8.751;MLEAC=1;MLEAF=0.500;MQ=57.87;MQ0=0;MQRankSum=-0.741;QD=10.80;ReadPosRankSum=0.365 GT:ADP:GQ:PL 0/1:4,6:10:99:147,0,124
    10 43159396 . G A 21.77 LowQual AC=2;AF=1.00;AN=2;DP=2;FS=0.000;MLEAC=2;MLEAF=1.00;MQ=60.00;MQ0=0;QD=10.88 GT:ADP:GQ:PL 1/1:0,2:2:6:49,6,0

Latest Articles

Collapse

  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM
  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
30 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
32 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
28 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-04-2024, 09:00 AM
0 responses
53 views
0 likes
Last Post seqadmin  
Working...
X