Hi,
Please advise on the quality of the libraries. There are 10 in total (library ID, cell type, IP type)
15 - cell type 1, input;
16 - cell type 1, IP #1;
18 - cell type 1, IP #2;
19 - cell type 1, IP #3;
20 - cell type 1, IP with IgG;
21 - cell type 2, input;
22 - cell type 2, IP #1;
23 - cell type 2, IP #2;
25 - cell type 2, IP #3;
27 - cell type 2, IP with IgG.
These libraries were assessed in the sequencing facility and failed according to their report. While it is understandable for some of the libraries, I do not understand why other libraries failed. I would like somebody to comment on the report files and perhaps to suggest what could be done.
For example, I can:
- prepare a library again;
- repeat size-selection;
- increase the library concentration (from the library stocks I have).
I have prepared files with images copied from the report files. The reports which were done on my site (tapestation) look a bit different from the sequencing facility reports. I am not sure why.
At the top of the image #1 I have copied a part of the report table with library DNA concentration measured in the sequencing facility.
Thank you!!!
Please advise on the quality of the libraries. There are 10 in total (library ID, cell type, IP type)
15 - cell type 1, input;
16 - cell type 1, IP #1;
18 - cell type 1, IP #2;
19 - cell type 1, IP #3;
20 - cell type 1, IP with IgG;
21 - cell type 2, input;
22 - cell type 2, IP #1;
23 - cell type 2, IP #2;
25 - cell type 2, IP #3;
27 - cell type 2, IP with IgG.
These libraries were assessed in the sequencing facility and failed according to their report. While it is understandable for some of the libraries, I do not understand why other libraries failed. I would like somebody to comment on the report files and perhaps to suggest what could be done.
For example, I can:
- prepare a library again;
- repeat size-selection;
- increase the library concentration (from the library stocks I have).
I have prepared files with images copied from the report files. The reports which were done on my site (tapestation) look a bit different from the sequencing facility reports. I am not sure why.
At the top of the image #1 I have copied a part of the report table with library DNA concentration measured in the sequencing facility.
Thank you!!!
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