Is indexing your .bam files a necessary step for running the samtools variant calling pipeline (ie samtools mpileup, bcftools view etc)?
I know that indexing your genome/transcriptome is a necessary step but I can;t figure out if my coordinate sorted .bam files need to be indexed as well.
Thank you!
I know that indexing your genome/transcriptome is a necessary step but I can;t figure out if my coordinate sorted .bam files need to be indexed as well.
Thank you!
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