Hi everyone,
this is my first post so, please be patient!
I'm trying to create a blast DB using the RNAseq data from ENCODE.
I've downloaded both the FASTQ reads and the .bam/bai files.
Is it possible to extract the fasta sequences of all the transcript from the BAM file?
Alternatively should i try to do a de novo assembly using Trinity?
Thanx a lot.
Regards,
Davide
this is my first post so, please be patient!
I'm trying to create a blast DB using the RNAseq data from ENCODE.
I've downloaded both the FASTQ reads and the .bam/bai files.
Is it possible to extract the fasta sequences of all the transcript from the BAM file?
Alternatively should i try to do a de novo assembly using Trinity?
Thanx a lot.
Regards,
Davide