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Old 12-14-2010, 03:56 AM   #1
HTFRIEDELFF
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Location: madrid

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Default NORMALIZATION Illumina DESeq

Dear colleagues

In my group, we are trying to give an answer to our results, but the first problem is normalization. We have used DESeq script for R and it´s greatĦĦĦ but we need to compare 3 independent samples without replicates, anybody knows how could we resolve it?

Thanks a lotĦĦ
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Old 12-15-2010, 01:47 AM   #2
Wolfgang Huber
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Dear Htfriedelff

have a look at section 6 of the DESeq vignette, "Working without any replicates".
Replicates do, however, tend to be important for biologically meaningful experiments, the DESeq software will not work any magic here.

Wolfgang
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