I have performed the same search using tblastx 2.2.25 and 2.2.24. With 2.2.24 I get sensible hits but with the latest version I get no hits.
Does anyone know if something changed with the latest version?
My command for both versions is
Does anyone know if something changed with the latest version?
My command for both versions is
Code:
tblastn -db ESTs -query ESTs_to_check.faa -out EST_hits.tab -outfmt 6 -num_threads 8