Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa

Similar Threads
Thread Thread Starter Forum Replies Last Post
Maximum number of cycles from 300-cycle MiSeq kit... ECO Illumina/Solexa 13 11-09-2012 05:51 AM
First Cycle Intensity Issues jpsmith Illumina/Solexa 1 10-06-2011 01:27 AM
Use Capture Library in Human all exon 50MB kit and other Reagents 38MB kit? shinne Sample Prep / Library Generation 2 09-25-2011 08:00 PM
Agilent SureSelect Human All Exon 50MB kit vs 38MB kit nimmi Sample Prep / Library Generation 3 11-30-2010 06:52 AM
How many cycles you can get out of the 36 cycle kit version 2? schönblick Illumina/Solexa 12 07-22-2009 05:34 AM

Thread Tools
Old 02-07-2012, 11:15 PM   #1
Junior Member
Location: San Jose, CA

Join Date: Feb 2012
Posts: 1
Default GA II - 36 cycle kit

We are trying to run a 40 cycle + 7 base index run and using one 36 cycle kit is not enough but two will be too much.

We are thinking to split one of the 36 cycle kit into 4x10 cycle kits which means it require to thawn/freeze some of the reagents. Has anyone done that before? Would you recommend doing it?

doofus is offline   Reply With Quote
Old 02-08-2012, 08:34 AM   #2
Location: St. Louis, MO

Join Date: Jun 2009
Posts: 23

We tried to freeze already thawed sequencing chemicals a couple years ago, and saw terrible quality metrics from that run.
hoisinjl is offline   Reply With Quote
Old 04-23-2012, 09:02 PM   #3
Junior Member
Location: Palo Alto, CA

Join Date: Apr 2012
Posts: 1

I have been running 40+7 cycles. I split one of 36 cycle kit into 4x10 cycle kits and re-freeze. I have done this for about 8 runs now, no problem. I have asked an FAS before I did this, he said it's fine.
ClownFishJokes is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 10:30 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO