Hi all.
I am trying to prepare several ChIP-seq libraries. I started with 3ng DNA, which was sonicated to 100-500bp length. After the blunt ending, A tail adding, and adapter ligation, I did the qPCR to do the quantitation but found most of the qPCR products were adaptor dimers (about 120bp). Does anybody have similar experiences or any suggestions? Thanks.
Z.
I am trying to prepare several ChIP-seq libraries. I started with 3ng DNA, which was sonicated to 100-500bp length. After the blunt ending, A tail adding, and adapter ligation, I did the qPCR to do the quantitation but found most of the qPCR products were adaptor dimers (about 120bp). Does anybody have similar experiences or any suggestions? Thanks.
Z.
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