SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
MiSeq Custom Sequencing Primer Tm? pmiguel Illumina/Solexa 43 11-12-2015 11:52 AM
Miseq Custom primer for 16s V1V3 region lmma Illumina/Solexa 12 02-26-2015 01:13 PM
MiSeq indexing amplicons and custom primer Tm melcar Illumina/Solexa 5 03-13-2014 04:48 PM
Short LNA custom sequencing primer for MiSeq bunce Illumina/Solexa 1 02-24-2014 01:14 AM
50+% of my HiSEQ reads are 3' primer (custom primer used) ZAAB Sample Prep / Library Generation 3 02-06-2012 11:00 AM

Reply
 
Thread Tools
Old 04-21-2015, 01:34 PM   #1
Cyrus Taheri
Junior Member
 
Location: Canada

Join Date: Aug 2014
Posts: 8
Question Miseq custom primer design, primer linker

Miseq custom primer design, primer linker

Many papers have used a 2bp primer linker in 5 end of the amplicon primers. As, I understand this is to prevent taxon specific PCR bias due to matches to the overhanger and/ or barcodes. I am wondering why 16S protocol did not add a linker.
Thanks,
Cyrus Taheri is offline   Reply With Quote
Old 04-21-2015, 05:25 PM   #2
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,232
Default

Good question for Illumina. Addition of linker does make sense with no known or presumed detrimental effect.
nucacidhunter is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 09:10 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO