Hi all,
I would like to know if somebody encountered the same problem as we do at the moment.
Since Mid February we are no longer able to spike our Fluidigm custom primers (CS1 and CS2) into the Illumina primers (Miseq V2 nano kit 500 cycles).
We tried now 4 times with different libraries and also with a library that worked before but got no results but PhiX reads.
When we used the CS1 and CS2 primers as custom primers alone the library was read as usual.
We tested the primers with our libraries in a normal pcr and they gave perfectly strong signals.
Thanks
I would like to know if somebody encountered the same problem as we do at the moment.
Since Mid February we are no longer able to spike our Fluidigm custom primers (CS1 and CS2) into the Illumina primers (Miseq V2 nano kit 500 cycles).
We tried now 4 times with different libraries and also with a library that worked before but got no results but PhiX reads.
When we used the CS1 and CS2 primers as custom primers alone the library was read as usual.
We tested the primers with our libraries in a normal pcr and they gave perfectly strong signals.
Thanks
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