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Old 05-29-2018, 12:22 PM   #1
cement_head
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Question RAD-Seq Library Loading Concentration?

Hello,

Would I be correct in assuming that RAD-Seq libraries should be treated as if they were low-diversity amplicon libraries (i.e. 16S rDNA/microbiome) for the purposes of loading? In other words, we typically load amplicons at 4.0 pM with a 10% PhiX spike-in - would the best practices be the same for the RAD-Seq libraries?

Thanks...
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Old 05-30-2018, 05:54 AM   #2
cement_head
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After digging around on the web last night; the answer seems to be "yes, treat them as per low-diversity libraries"...
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