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Old 07-12-2011, 07:50 AM   #1
Location: Maryland

Join Date: May 2011
Posts: 66
Default Intra-sample variability, Illumina TruSeq mRNA

Does anyone know if there is a technical issue during either mRNA library prep or data handling that could cause two libraries prepared from the same cell population to look radically different? We have made multiple libraries from the same sample and the results appear quite discouraging. We're not sure if we're doing something wrong with analysis, or whether there was a problem with sample prep. Anyone know of any common pitfalls that could explain our problem?
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