Hi everybody,
I am designing a novel pipeline for processing metagenomic datasets. I'd like to use for functional annotation the following methods:
1) Full domain alignment methods (e.g. Pfam and TIGRFam)
2) Multiple motif methods (PRINTS)
3) Single motif methods (ProSite)
I guess that this list goes from very specific methods (1-->Pfam) to very sensitive methods (3-->ProSite), right? Thus, do you recommend me to analyse my sequences with Pfam first, then to analyse the remaining unannotated sequences with PRINTS and finishing with ProSite?
I cannot see the differences between Pfam and TIGRFam.
Any suggestion?
Thanks!
I am designing a novel pipeline for processing metagenomic datasets. I'd like to use for functional annotation the following methods:
1) Full domain alignment methods (e.g. Pfam and TIGRFam)
2) Multiple motif methods (PRINTS)
3) Single motif methods (ProSite)
I guess that this list goes from very specific methods (1-->Pfam) to very sensitive methods (3-->ProSite), right? Thus, do you recommend me to analyse my sequences with Pfam first, then to analyse the remaining unannotated sequences with PRINTS and finishing with ProSite?
I cannot see the differences between Pfam and TIGRFam.
Any suggestion?
Thanks!
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