We have 96 gut microbiome samples that we want to run using the Nextera XT or Nextera Kit. Most of our samples have between 1 - 40 ng of DNA. Would it be best to dilute our samples, we dont know how that would affect library quality. We want to run it on the HiSeq 2500 but a previous thread said that the bead normalizing step would be skipped and qPCR was recommended by Illumina (which is best for us since we are limited by budget and we would have to purchase the Amp XP beads and a magnetic plate). Or would it be better to use the TrueSeq kit? (first time doing library prep)
Best,
Maria
Best,
Maria
Comment