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Looking for Aligner for mapping insertions WindGirl2008 Bioinformatics 5 06-23-2010 05:25 PM

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Old 04-19-2018, 06:37 AM   #1
yueli
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Default best suitable aligner for RNA-seq mapping

Hello,

Human RNA-seq dataset was generated from Illumina HiSeq 3000 with 2X100 cycles run.

The first step is making alignment of the reads to the human genome. These are many aligner, such as: Bowtie, GASSST, PASS, SOAP, BOAT. Each aligners has different performs in different kinds of data.

Which is the best suitable aligner for RNA-seq data?

Thank you in advance for great help!

Sincerely,

Yue
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Old 04-19-2018, 06:47 AM   #2
GenoMax
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There is no "best" aligner in a practical sense. Most aligners will give you reasonable alignments that you should be able to use for expression analysis.

You want to make sure the aligner you use is "splice-aware" since you have human data. BBMap, STAR, HISAT2 are examples of such aligners.
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Old 04-19-2018, 07:16 AM   #3
yueli
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Thanks for GenoMax response!
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aligner, genome, illumina, mapping, rna-seq

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