Hi everyone,
I am kind of new to rna seq and mostly to 10x genomics. I got some data from a 10x genomics and in contrast to Hiseq/Nextseq etc, I have 4 types of files.
I have R1,R2,R3 and I1 files. I am a bit confused about what is what. So R1 has reads of 114 length so it should be my sequence. The R2 files have sequences of 14bp (is that the 10x barcode ?)
R3 contain sequences of 10 bp (UMI ?) and I1 should be sample barcodes
Could someone help me clear this out ?
I am kind of new to rna seq and mostly to 10x genomics. I got some data from a 10x genomics and in contrast to Hiseq/Nextseq etc, I have 4 types of files.
I have R1,R2,R3 and I1 files. I am a bit confused about what is what. So R1 has reads of 114 length so it should be my sequence. The R2 files have sequences of 14bp (is that the 10x barcode ?)
R3 contain sequences of 10 bp (UMI ?) and I1 should be sample barcodes
Could someone help me clear this out ?
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