High-Throughput DNA Shearing

ABSOLiDuser · 01-22-2009, 11:10 PM

Hello,

Does anybody have any experience or recommendation about DNA shearing tools that can handle multiple samples simultaneously? I know there are some systems out there that can sonicate a whole plate. Has anybody tried them? Also, I have seen several posts on the Bioruptor: does anybody know if it can go down to 150-200bp peak size? How long does it take?

Thank you so much!

EPICENTRE · 03-04-2009, 08:20 AM

We are working on a way to fragment and add adaptors using a transposon-based method. Our fragments tend to smear in the 150-2000 bp range. How critical is it for you to be in the 150-200 bp range? We can gel purify, but would like to eliminate this step if possible. Which adaptors do you need to add?

ABSOLiDuser · 03-05-2009, 10:50 PM

Thanks EPI.

sci_guy · 03-08-2009, 06:28 PM

Hi,

I believe ABI is working on a 96 well sonicator for SOLiD called a 'Piranha'. I heard something about it when I attended a SOLiD V3 launch seminar. Talk to your rep and see if this is the case.

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01-22-2009, 11:10 PM	#1
ABSOLiDuser Junior Member Location: California Join Date: Jan 2009 Posts: 2	High-Throughput DNA Shearing Hello, Does anybody have any experience or recommendation about DNA shearing tools that can handle multiple samples simultaneously? I know there are some systems out there that can sonicate a whole plate. Has anybody tried them? Also, I have seen several posts on the Bioruptor: does anybody know if it can go down to 150-200bp peak size? How long does it take? Thank you so much! Last edited by ABSOLiDuser; 03-09-2009 at 08:35 PM.

03-05-2009, 10:50 PM	#3
ABSOLiDuser Junior Member Location: California Join Date: Jan 2009 Posts: 2	Thanks EPI. Last edited by ABSOLiDuser; 03-09-2009 at 08:34 PM.

03-08-2009, 06:28 PM	#4
sci_guy Member Location: Sydney, Australia Join Date: Jan 2008 Posts: 83	Piranha Hi, I believe ABI is working on a 96 well sonicator for SOLiD called a 'Piranha'. I heard something about it when I attended a SOLiD V3 launch seminar. Talk to your rep and see if this is the case.

03-04-2009, 08:20 AM	#2
EPICENTRE Registered Vendor Location: Madison Join Date: Mar 2009 Posts: 5	We are working on a way to fragment and add adaptors using a transposon-based method. Our fragments tend to smear in the 150-2000 bp range. How critical is it for you to be in the 150-200 bp range? We can gel purify, but would like to eliminate this step if possible. Which adaptors do you need to add?