Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Prettyprint and SHRiMP 2.2.2

    Hello,

    I am having an issue with the pretty print function in SHRiMP 2.2.2.

    I have several scripts for working through the pipeline (that work for 2.1.1b).

    With 2.2.2, I use gmapper-cs, then some filtering, then use probcalc, and finally I want to use prettyprint-cs. However, I get this error:

    Code:
    Loading shrimp output file...
    Loaded 11537 alignments from shrimp output (11537 unique reads)
    Loading read file(s)...
    Loaded 11537 colourspace reads in 1 file(s) (415332 total bases)
    Loading genome contig file(s)...
    warning: score differs from input file (read="862_925_542", genome="Bd1_100805_100840_F")
             Most likely cause is that prettyprint does not use anchors.
    *** glibc detected *** double free or corruption (out): 0x000000000970fc80 ***
    /share/sge6.2/execd_spool//i182-110/job_scripts/2376614: line 11:  1506 Aborted
    I make sure to use the --shrimp-format flag for gmapper-cs. Anyone have any insights here? If I run gmapper with the -p flag it WILL prettyprint (but I need to do some more filtering before this step).

    Appreciate it.

  • #2
    Hmm you know what... never mind... I would just delete this post, but you may find this useful. The newest version of SHRiMP does not fix the terminal gaps issue, so it really gains us nothing (at least for my purposes). Thus, we will continue to use version 2.1.1b

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Current Approaches to Protein Sequencing
      by seqadmin


      Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
      04-04-2024, 04:25 PM
    • seqadmin
      Strategies for Sequencing Challenging Samples
      by seqadmin


      Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
      03-22-2024, 06:39 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 04-11-2024, 12:08 PM
    0 responses
    25 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 10:19 PM
    0 responses
    28 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 09:21 AM
    0 responses
    24 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-04-2024, 09:00 AM
    0 responses
    52 views
    0 likes
    Last Post seqadmin  
    Working...
    X