I'm getting lots of conflicting results when quantifying my libraries prior to cluster generation. Recently I've had lots of samples that exhibit the following after enrichment PCR:
By eye, agarose gel shows nice bright band, correct size and intensity for a tenfold concentration.
Nanodrop reads ~10ng/ul
Bioanalyzer DNA1000 chip sees nothing at all, or .1-.2ng/ul
Bioanalyzer high-sensitivity chip sees ~1-2ng/ul.
I'm planning on running all these with a known standard to figure out who is closest, but recently I've read some no-so-good stuff about the bioanalyzer and it's ability to determine concentration. Has anyone else had this experience?
Thanks!
By eye, agarose gel shows nice bright band, correct size and intensity for a tenfold concentration.
Nanodrop reads ~10ng/ul
Bioanalyzer DNA1000 chip sees nothing at all, or .1-.2ng/ul
Bioanalyzer high-sensitivity chip sees ~1-2ng/ul.
I'm planning on running all these with a known standard to figure out who is closest, but recently I've read some no-so-good stuff about the bioanalyzer and it's ability to determine concentration. Has anyone else had this experience?
Thanks!
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