Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Why is Sanger more accurate than NGS?

    Hi All,

    I have heard that sanger/dye termination sequencing is more accurate than illumina/solexa DNA cluster-based sequencing. I have been reading about both methods and am trying to identify the key point(s) in the illumina/solexa process in which additional error is introduced and accuracy diverges from the sanger method. I read that it is because illumina/solexa sequencing ‘requires PCR amplification’ of DNA clusters.
    Is cluster amplification the main error-introducing step?
    Why is this step necessary for the process to work, i.e. why can’t the incorporated nucleotide signal be captured while only a single molecule is being synthesized?

    Thank you!
    kateveronica

  • #2
    Here is one resource that talks about this issue:

    Comment


    • #3
      Fundamentally, Sanger sequencing is equivalent to sequencing a single amplicon on an NGS and aggregating that information to a "consensus".

      That level of redundancy buys the "accuracy" of Sanger sequencing.

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Strategies for Sequencing Challenging Samples
        by seqadmin


        Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
        03-22-2024, 06:39 AM
      • seqadmin
        Techniques and Challenges in Conservation Genomics
        by seqadmin



        The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

        Avian Conservation
        Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
        03-08-2024, 10:41 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, Yesterday, 06:37 PM
      0 responses
      10 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, Yesterday, 06:07 PM
      0 responses
      10 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-22-2024, 10:03 AM
      0 responses
      51 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-21-2024, 07:32 AM
      0 responses
      67 views
      0 likes
      Last Post seqadmin  
      Working...
      X