Considering that rRNA constituted 99% of your sample, 50-70 ng of rRNA-depleted RNA is in line with the expected yield. This would be sufficient to prepare an RNA-Seq library. For example, our ScriptSeq v2 library prep kit requires 500 pg to 50 ng input RNA (either poly(A) or rRNA-depleted).
What method are you using to quantify the RNA after Ribo-Zero treatment?
What method are you using to quantify the RNA after Ribo-Zero treatment?
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