Hi,
I want to use TargetScan locally on my computer. So I downloaded TargetScan ( http://www.targetscan.org/cgi-bin/ta...cgi?db=vert_50) .
The input is composed like this :
The script takes two input files
1) A tab-delimited file that lists the miRNA seed sequences.
2) A tab-delimited multiple sequence alignment of the 3' UTRs of genes from the desired species.
The first point is ok. The second I don't know how produce such an alignment (what is the query ? ) . I've the 3' UTR of the organism I want to analyze ( in this research a virus ).
Anyone can help me ?
Thanks a lot,
Nicolas
I want to use TargetScan locally on my computer. So I downloaded TargetScan ( http://www.targetscan.org/cgi-bin/ta...cgi?db=vert_50) .
The input is composed like this :
The script takes two input files
1) A tab-delimited file that lists the miRNA seed sequences.
2) A tab-delimited multiple sequence alignment of the 3' UTRs of genes from the desired species.
The first point is ok. The second I don't know how produce such an alignment (what is the query ? ) . I've the 3' UTR of the organism I want to analyze ( in this research a virus ).
Anyone can help me ?
Thanks a lot,
Nicolas
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