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  • Unwanted Small (~80bp) Bioanalyzer Peaks

    After PCR enriching some libraries I noticed small peaks of 80 bp in all of my samples (see image). I am using NEB's ChIP-seq kit for Illumina. My inserts are ~65 bp. I see this in my negative control too.

    Reading around on the forum it seems that these are likely unreacted/excess primer. I would size select but I'm worried about sample loss since my peak is so small. Do I need to worry about them hybridizing to the flowcell?

    If anyone has some insight I'd much appreciate it. Thanks.
    Attached Files
    Last edited by Daytwa; 04-13-2013, 06:06 PM. Reason: Forgot attachment

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