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Hello everybody,
For my thesis I have some RNA-Seq human data (control,treatment) and My software is CLC Genomics.
Currently I do not want to detect novel lncRNA, only I want to identify differentially expressed lncRNA and then compare them with set up experiment. (The same way I do for genes)
I want to know may I use RNA-Seq data (after trimming adapter and cleaning low quality sequences) for identifying differentially expressed lncRNA by RNA-Seq analysis immediately or I should do read preparation?
I mean could I get started RNA-Seq analysis and use one ref (Genecode.v19.lncRNA_ transcripts) for identifying differentially expressed lncRNA and then set up an experiment?
For my thesis I have some RNA-Seq human data (control,treatment) and My software is CLC Genomics.
Currently I do not want to detect novel lncRNA, only I want to identify differentially expressed lncRNA and then compare them with set up experiment. (The same way I do for genes)
I want to know may I use RNA-Seq data (after trimming adapter and cleaning low quality sequences) for identifying differentially expressed lncRNA by RNA-Seq analysis immediately or I should do read preparation?
I mean could I get started RNA-Seq analysis and use one ref (Genecode.v19.lncRNA_ transcripts) for identifying differentially expressed lncRNA and then set up an experiment?