Hi all,
My lab has recently started using 454 GS FLX titanium pyroseqeuncing of cDNAs for several transcriptome projects with non-model organisms. All of our data has been assembled by Newbler 2.3 and later annotated by search against BLASTx nr database.
We are debating how we should refer to the assembled "isogroups" in our paper. Is appropriate to refer to an "isogroup" as a gene? Or a 'gene'? What about a isotigs or contigs that are not assembled into isogroups but are annotated?
Thanks for you help!
My lab has recently started using 454 GS FLX titanium pyroseqeuncing of cDNAs for several transcriptome projects with non-model organisms. All of our data has been assembled by Newbler 2.3 and later annotated by search against BLASTx nr database.
We are debating how we should refer to the assembled "isogroups" in our paper. Is appropriate to refer to an "isogroup" as a gene? Or a 'gene'? What about a isotigs or contigs that are not assembled into isogroups but are annotated?
Thanks for you help!
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