Hello,
We are validating our Illumina machine. Several samples from mouse ChIP sequencing show consistently an excess of AT (60%) vs CG across the read length. It may be related to sequence composition, of course.
However I want to ask:
Which factors/errors during sample preparation, run and basic analysis can produce a consistent excess of AT nucleotides?
We are validating our Illumina machine. Several samples from mouse ChIP sequencing show consistently an excess of AT (60%) vs CG across the read length. It may be related to sequence composition, of course.
However I want to ask:
Which factors/errors during sample preparation, run and basic analysis can produce a consistent excess of AT nucleotides?