Does anyone know of any good methods for multiplexing more than 384 samples on a MiSeq run? The Nextera XT Index Kit allows indexing for a maximum of 384 samples. We have already purchased one of these kits, but would like to add more samples onto our run. I know the Illumina customer letter provided sequences for some of their barcodes, but not for all of them. Does anyone know if the rest of these are available elsewhere? I suppose it would be possible to order oligos identical to the Nextera barcode adaptors but with different 8 bp barcode sequences. Any thoughts?
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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04-04-2024, 04:25 PM -
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