Recommendations for sequencing facility

crh · 04-09-2011, 07:34 AM

Hi
Can you suggest companies that do RNAseq services? We would like to propose a project and I’d like to get some recommendations for good quality sequencing at a reasonable price.

thanks

Charles

jo_mason · 04-13-2011, 07:16 PM

Hi Charles,
I'd be happy to do a quote for you. Although our sequencer is fairly tied up this month, next month we have several RNASeq projects running, so we would try and give you a good price.
Send me some details of your project and I'll see what we can do for you.
my email is: joanne@mgrc.com.my
The company website if you want to have a look is www.mgrc.com.my
Best wishes,
Jo

jbertsche · 04-22-2011, 05:16 AM

Hi Charles,

I'd also be more than happy to write up a quote for you. We use the 454 platform for our cDNA sequencing. My email is jbertsche@aibiotech.com. Send me a little info about the project and we'll see if we can't work something out.

The company website is: www.aibiotech.com

Regards,
Joe Bertsche

JackieBadger · 04-22-2011, 11:52 AM

What ever you do, DO NOT go with EURO FINS|OPERON !!!!

honey · 04-22-2011, 09:57 PM

You may be better of getting it done from some Univ core and some of them also have bionformatics support at nominal charges.

monad · 05-08-2011, 05:23 PM

try newly organized genome center, global genome research institute, www.ggri.org

Pricing is one thing. They will consult with you first to figue out the most efficient experimental design and important considerations before contract you.

DeWalchNGS · 05-25-2011, 02:43 PM

Quote:

Originally Posted by crh

Hi
Can you suggest companies that do RNAseq services? We would like to propose a project and I’d like to get some recommendations for good quality sequencing at a reasonable price.

thanks

Charles

Hi Charles,

I would be happy to provide a quote as well! Please email me at lpatty@dewalch.com.

Lindsay Patty
DeWalch Technologies
ls.dewalch.com

james hadfield · 05-27-2011, 02:16 PM

check out the google Map there are lots of providers on there. I suspect what users want is a way to see who can give back high quality data!

kamichailo · 06-15-2011, 12:54 PM

Charles:

Expression Analysis (EA) provides high quality RNA Seq services. We are experiencing 110 million reads, consistently, compared to illumina's specs of 60 million reads. We also have an analysis algorythm which enables us to perform alignment and achieve up to 99% alignment. www.expressionanalysis.com

monad · 06-15-2011, 02:13 PM

We can get as high as 130M reads per lane of HiSeq. However, your target should be more close to 100M passing reads (or not to exceed much beyond 100M) as HiSeq is more sensitive to high density clusters and you will get diminishing return at certain density. Passing rate at high density seems dependent on library type and quality.

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04-09-2011, 07:34 AM	#1
crh Member Location: tx Join Date: Dec 2009 Posts: 46	Recommendations for sequencing facility Hi Can you suggest companies that do RNAseq services? We would like to propose a project and I’d like to get some recommendations for good quality sequencing at a reasonable price. thanks Charles

04-13-2011, 07:16 PM	#2
jo_mason Member Location: Malaysia Join Date: Dec 2010 Posts: 20	Hi Charles, I'd be happy to do a quote for you. Although our sequencer is fairly tied up this month, next month we have several RNASeq projects running, so we would try and give you a good price. Send me some details of your project and I'll see what we can do for you. my email is: joanne@mgrc.com.my The company website if you want to have a look is www.mgrc.com.my Best wishes, Jo __________________ Jo Mason joanne@mgrc.com.my

04-22-2011, 09:57 PM	#5
honey Senior Member Location: Pittsburgh Join Date: Feb 2010 Posts: 151	Option You may be better of getting it done from some Univ core and some of them also have bionformatics support at nominal charges.

04-22-2011, 05:16 AM	#3
jbertsche Junior Member Location: Virginia Join Date: Mar 2011 Posts: 2	Hi Charles, I'd also be more than happy to write up a quote for you. We use the 454 platform for our cDNA sequencing. My email is jbertsche@aibiotech.com. Send me a little info about the project and we'll see if we can't work something out. The company website is: www.aibiotech.com Regards, Joe Bertsche

04-22-2011, 11:52 AM	#4
JackieBadger Senior Member Location: Halifax, Nova Scotia Join Date: Mar 2009 Posts: 381	What ever you do, DO NOT go with EURO FINS\|OPERON !!!!

05-08-2011, 05:23 PM	#6
monad Member Location: California Join Date: May 2008 Posts: 40	try newly organized genome center, global genome research institute, www.ggri.org Pricing is one thing. They will consult with you first to figue out the most efficient experimental design and important considerations before contract you.

05-27-2011, 02:16 PM	#8
james hadfield Moderator Cambridge, UK Community Forum Location: Cambridge, UK Join Date: Feb 2008 Posts: 221	check out the google Map there are lots of providers on there. I suspect what users want is a way to see who can give back high quality data!

06-15-2011, 12:54 PM	#9
kamichailo Junior Member Location: Raleigh Join Date: Mar 2010 Posts: 8	Charles: Expression Analysis (EA) provides high quality RNA Seq services. We are experiencing 110 million reads, consistently, compared to illumina's specs of 60 million reads. We also have an analysis algorythm which enables us to perform alignment and achieve up to 99% alignment. www.expressionanalysis.com

06-15-2011, 02:13 PM	#10
monad Member Location: California Join Date: May 2008 Posts: 40	We can get as high as 130M reads per lane of HiSeq. However, your target should be more close to 100M passing reads (or not to exceed much beyond 100M) as HiSeq is more sensitive to high density clusters and you will get diminishing return at certain density. Passing rate at high density seems dependent on library type and quality.