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06-27-2011, 10:07 AM
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#1 |
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Junior Member
Location: Curitiba, Brazil Join Date: Jun 2011
Posts: 4
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Changing the read length after the run had started
Hellow,
I started a transcriptome barcoding run in SOLiD4. After the barcoding sequencing was finish I realized that the read length for tag F3 was 5 bp instead of 50 bp. I would like to know if someone had this problem and if is possible to change the read length in this stage. Thanks all. |
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06-27-2011, 12:18 PM
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#2 |
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Senior Member
Location: Purdue University, West Lafayette, Indiana Join Date: Aug 2008
Posts: 2,317
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Yes, but it is not trivial. We had this happen once. Applied Biosystems had a Bioinformatics Field Specialist remotely modify the run to the correct number of bases.
Not a minor undertaking, though. -- Phillip |
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