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Old 11-08-2011, 05:38 PM   #1
Location: USA

Join Date: Jul 2010
Posts: 58
Default Library prep for 50ng input with TruSeq DNA- or RNAseq adapters?

Dear all,

I am going to process library preparation with 50 ng DNA. I am just concerning ratio of adaptersNA input. Thus, I am considering to use adapters from TruSeq RNAseq kit, which has lower molarity adapters. Has anyone done library prep with lower DNA input?
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Old 11-09-2011, 05:03 AM   #2
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,317

Yes or you could just dilute your DNA TruSeq adapters 20x.

We have gone below 1 ug. Possibly to 100 ng, with no modifications to the protocol. But we generally size select prior to enrichment PCR. So adapter dimers are not that much of an issue.

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