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Old 02-22-2012, 06:09 PM   #1
Junior Member
Location: Shanghai, China

Join Date: Dec 2011
Posts: 1
Question Minimum coverage needed of de novo small RNA sequencing


I am a novice member with some novice questions.
I have data about small RNA profile of an avian species without reference genome. I get less than 1M reads per sample after filtration. My questions are: 1, how many reads per sample should be enough? 2, for analysis, should I map them to an related species' genome?
Also, I am looking for publications about de novo in small RNA.

Thanks a lot in advance for your help!
hechanner is offline   Reply With Quote

coverage, small rna

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