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02-22-2012, 06:09 PM
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Junior Member
Location: Shanghai, China Join Date: Dec 2011
Posts: 1
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Minimum coverage needed of de novo small RNA sequencing
Hello,
I am a novice member with some novice questions.  I have data about small RNA profile of an avian species without reference genome. I get less than 1M reads per sample after filtration. My questions are: 1, how many reads per sample should be enough? 2, for analysis, should I map them to an related species' genome? Also, I am looking for publications about de novo in small RNA. Thanks a lot in advance for your help! |
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| coverage, small rna |
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