transposon/insertion sequencing techniques

lvenga · 01-31-2013, 07:41 AM

Hi,

could anyone briefly summarise the main differences between:

-IN-Seq (Insertion sequencing)
-Tn-Seq (Transposon sequencing)
-HITS (High-throughput insertion tracking sequencing)
-TraDIS (Transposon-directed insertion-site sequencing)
?

Thanks a lot!

dcnadler · 05-30-2013, 01:17 PM

Quote:

Originally Posted by lvenga

Hi,

could anyone briefly summarise the main differences between:

-IN-Seq (Insertion sequencing)
-Tn-Seq (Transposon sequencing)
-HITS (High-throughput insertion tracking sequencing)
-TraDIS (Transposon-directed insertion-site sequencing)
?

Thanks a lot!

There is a review that just came out which should provide the answer: http://www.nature.com/nrmicro/journa...micro3033.html

It has a section that briefly describes the differences and a nice figure to illustrate them.

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01-31-2013, 07:41 AM	#1
lvenga Junior Member Location: london Join Date: Jan 2013 Posts: 1	transposon/insertion sequencing techniques Hi, could anyone briefly summarise the main differences between: -IN-Seq (Insertion sequencing) -Tn-Seq (Transposon sequencing) -HITS (High-throughput insertion tracking sequencing) -TraDIS (Transposon-directed insertion-site sequencing) ? Thanks a lot!