Platform comparison of read lengths

ryantkoehler · 10-05-2009, 09:37 AM

Hi all

Does anyone know of a good comparison of next gen sequencing platform read specs? In particular, I'm interested in common/maximal read lengths, mate-pair insert ranges, and ideally, error rate ranges and/or estimates and modes (i.e. number of wrong base(s) read from source sequence and type of error; indel, substitution, etc). Of course it would be nice to know advertised throughput, costs (capital and operational), other pros, cons, and features, as well :-)

I found it surprisingly difficult to find comparable read length numbers when I was compiling a table for a genomic sequencing class I taught this summer. I shared the values I found with a big caveat that these are moving targets and from different sources. My students uncovered several papers with numbers but I suspect some of these are already outdated and current numbers have changed.

1) Shendure J, Ji H. Next-generation DNA sequencing. Nat Biotechnol. 2008 Oct;26(10):1135-45.

2) Ansorge WJ. Next-generation DNA sequencing techniques. N Biotechnol. 2009 Apr;25(4):195-203. Epub 2009 Feb 3.Click here to read

Table 1 in the Shendure paper is a good (outdated!) model for what I'm looking for. It lists company, technology, costs, errors, read lengths. The attached picture is a screenshot of this table.

If anyone knows of newer numbers that would great, especially if they are already tabulated together!

Thanks,
-Ryan

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10-05-2009, 09:37 AM	#1
ryantkoehler Junior Member Location: Portland, OR Join Date: Aug 2009 Posts: 7	Platform comparison of read lengths Hi all Does anyone know of a good comparison of next gen sequencing platform read specs? In particular, I'm interested in common/maximal read lengths, mate-pair insert ranges, and ideally, error rate ranges and/or estimates and modes (i.e. number of wrong base(s) read from source sequence and type of error; indel, substitution, etc). Of course it would be nice to know advertised throughput, costs (capital and operational), other pros, cons, and features, as well :-) I found it surprisingly difficult to find comparable read length numbers when I was compiling a table for a genomic sequencing class I taught this summer. I shared the values I found with a big caveat that these are moving targets and from different sources. My students uncovered several papers with numbers but I suspect some of these are already outdated and current numbers have changed. 1) Shendure J, Ji H. Next-generation DNA sequencing. Nat Biotechnol. 2008 Oct;26(10):1135-45. 2) Ansorge WJ. Next-generation DNA sequencing techniques. N Biotechnol. 2009 Apr;25(4):195-203. Epub 2009 Feb 3.Click here to read Table 1 in the Shendure paper is a good (outdated!) model for what I'm looking for. It lists company, technology, costs, errors, read lengths. The attached picture is a screenshot of this table. If anyone knows of newer numbers that would great, especially if they are already tabulated together! Thanks, -Ryan