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Old 02-19-2013, 12:47 PM   #1
barturas
Junior Member
 
Location: Lithuania

Join Date: Jan 2013
Posts: 2
Default TruSeq lib prep anomaly

hello,
I encountered some weird thing after my library amplification with classical illumina library prep.
I went through size selection with sybrgold agarose gel, did analysis on Agilent Bioanalyzer, and got expected peaks.


After that I did standart illumina library amplification (10cycles), did again Agilent analysis and beside expected peak I got some weird peak in region of 3kb.


Has anyone any ideas? Is it some kind of chimeric entities emerged during PCR? This was E.coli gDNA.

Thanks!
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