Go Back   SEQanswers > General

Similar Threads
Thread Thread Starter Forum Replies Last Post
lib prep using SPRIworks sgupta Illumina/Solexa 12 03-09-2012 07:39 AM
Do proteins interfere with NGS or lib prep?? hmviit Sample Prep / Library Generation 1 05-08-2011 07:05 AM
SPRI lib prep? BIG_SNP Illumina/Solexa 32 03-23-2011 09:42 AM
Lib prep automation? BIG_SNP Illumina/Solexa 9 11-24-2010 08:20 AM
NEB lib prep kit BIG_SNP Illumina/Solexa 0 08-20-2009 03:34 PM

Thread Tools
Old 02-19-2013, 12:47 PM   #1
Junior Member
Location: Lithuania

Join Date: Jan 2013
Posts: 2
Default TruSeq lib prep anomaly

I encountered some weird thing after my library amplification with classical illumina library prep.
I went through size selection with sybrgold agarose gel, did analysis on Agilent Bioanalyzer, and got expected peaks.

After that I did standart illumina library amplification (10cycles), did again Agilent analysis and beside expected peak I got some weird peak in region of 3kb.

Has anyone any ideas? Is it some kind of chimeric entities emerged during PCR? This was E.coli gDNA.

barturas is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 10:43 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO