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Old 05-07-2013, 11:42 AM   #1
Francy87
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Default RNA storage and shipping for Illumina seq

Hello!
I need to send some samples to USA for mRNA seq. Since I can't prepare the cDNA here, I need to send the RNA. I thought to precipitate it and then send at 4C. Anybody has never done this? Or anybody has alternative options?
Moreover, someone knows which carrier is the best in mRNA precipitation (acrylamide or glycogen)?? Can they interfere with the following protocol?
Many thanks
Francesca
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Old 05-07-2013, 05:03 PM   #2
nkaushik
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The best will be to send total RNA in 0.1M soduim acetate and 2 vol ethanol. I used to send total RNA to US for Affymetrix and Exiqon microarray experiments.

Hope this helps

Narendra
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Old 05-07-2013, 06:10 PM   #3
kcchan
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If you have not already extracted your RNA, you can also lyse the samples in Trizol and ship the samples while frozen in Trizol. If the other lab is unable to perform a precipitation, you can also use RNAStable to store your sample. Your samples can then be shipped without dry ice and rehydration is as simple as adding water. The protocol is a bit more complicated for you, but it's much easier for the end user.
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Old 05-07-2013, 07:54 PM   #4
Francy87
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Hi! Which brand produce RNA stable? Do you know if it has been used for RNA for Illumina seq?
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Old 05-07-2013, 07:59 PM   #5
Francy87
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Quote:
Originally Posted by nkaushik View Post
The best will be to send total RNA in 0.1M soduim acetate and 2 vol ethanol. I used to send total RNA to US for Affymetrix and Exiqon microarray experiments.

Hope this helps

Narendra


Hi Narendra!
I need to follow up the protocol until the mRNA purification from rRNA, since the purification hasn't been paid to the seq facility... Do you know if the same way could be good also for mRNA? And do you meant shipping it in solution of sodium acetate and ethanol, without pelleting? At wich temperature did you ship?
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Old 05-07-2013, 09:38 PM   #6
kcchan
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Quote:
Originally Posted by Francy87 View Post
Hi! Which brand produce RNA stable? Do you know if it has been used for RNA for Illumina seq?
RNA Stable is produced by a company called Biomatrica. We've used it before to send total RNA, mRNA, and even small RNA for Illumina RNA library generation and didn't have any issues.
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Old 03-09-2015, 04:02 PM   #7
kclueneberg
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Hello,

Has anyone else have use RNAstable, what are your experiences with this product?

Thanks

Kind regards

Kathia
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Old 03-10-2015, 05:10 AM   #8
MU Core
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Our facility has used the RNA stabilization product from Gentegra with success. Samples are shipped at room temperature and remain stable. Upon arrival the RNA is resuspended and suitable for downstream applications such as an RNA-Seq library.

http://www.gentegra.com/
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Old 03-10-2015, 07:22 AM   #9
kmcarr
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Quote:
Originally Posted by MU Core View Post
Our facility has used the RNA stabilization product from Gentegra with success. Samples are shipped at room temperature and remain stable. Upon arrival the RNA is resuspended and suitable for downstream applications such as an RNA-Seq library.

http://www.gentegra.com/
What is the cost per sample for GenTegra-RNA?
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Old 03-10-2015, 07:49 AM   #10
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The cost through the US distributor is ~$4.00 per sample. There are 96-well plate options that reduce per sample cost below $2.00.

http://www.gentegra.com/distributor.html
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