RNA storage and shipping for Illumina seq

Francy87 · 05-07-2013, 11:42 AM

Hello!
I need to send some samples to USA for mRNA seq. Since I can't prepare the cDNA here, I need to send the RNA. I thought to precipitate it and then send at 4°C. Anybody has never done this? Or anybody has alternative options?
Moreover, someone knows which carrier is the best in mRNA precipitation (acrylamide or glycogen)?? Can they interfere with the following protocol?
Many thanks
Francesca

nkaushik · 05-07-2013, 05:03 PM

The best will be to send total RNA in 0.1M soduim acetate and 2 vol ethanol. I used to send total RNA to US for Affymetrix and Exiqon microarray experiments.

Hope this helps

Narendra

kcchan · 05-07-2013, 06:10 PM

If you have not already extracted your RNA, you can also lyse the samples in Trizol and ship the samples while frozen in Trizol. If the other lab is unable to perform a precipitation, you can also use RNAStable to store your sample. Your samples can then be shipped without dry ice and rehydration is as simple as adding water. The protocol is a bit more complicated for you, but it's much easier for the end user.

Francy87 · 05-07-2013, 07:54 PM

Hi! Which brand produce RNA stable? Do you know if it has been used for RNA for Illumina seq?

Francy87 · 05-07-2013, 07:59 PM

Quote:

Originally Posted by nkaushik

The best will be to send total RNA in 0.1M soduim acetate and 2 vol ethanol. I used to send total RNA to US for Affymetrix and Exiqon microarray experiments.

Hope this helps

Narendra

Hi Narendra!
I need to follow up the protocol until the mRNA purification from rRNA, since the purification hasn't been paid to the seq facility... Do you know if the same way could be good also for mRNA? And do you meant shipping it in solution of sodium acetate and ethanol, without pelleting? At wich temperature did you ship?

kcchan · 05-07-2013, 09:38 PM

Quote:

Originally Posted by Francy87

Hi! Which brand produce RNA stable? Do you know if it has been used for RNA for Illumina seq?

RNA Stable is produced by a company called Biomatrica. We've used it before to send total RNA, mRNA, and even small RNA for Illumina RNA library generation and didn't have any issues.

kclueneberg · 03-09-2015, 04:02 PM

Hello,

Has anyone else have use RNAstable, what are your experiences with this product?

Thanks

Kind regards

Kathia

MU Core · 03-10-2015, 05:10 AM

Our facility has used the RNA stabilization product from Gentegra with success. Samples are shipped at room temperature and remain stable. Upon arrival the RNA is resuspended and suitable for downstream applications such as an RNA-Seq library.

http://www.gentegra.com/

kmcarr · 03-10-2015, 07:22 AM

Quote:

Originally Posted by MU Core

Our facility has used the RNA stabilization product from Gentegra with success. Samples are shipped at room temperature and remain stable. Upon arrival the RNA is resuspended and suitable for downstream applications such as an RNA-Seq library.

http://www.gentegra.com/

What is the cost per sample for GenTegra-RNA?

MU Core · 03-10-2015, 07:49 AM

The cost through the US distributor is ~$4.00 per sample. There are 96-well plate options that reduce per sample cost below $2.00.

http://www.gentegra.com/distributor.html

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05-07-2013, 11:42 AM	#1
Francy87 Member Location: Italy Join Date: May 2013 Posts: 28	RNA storage and shipping for Illumina seq Hello! I need to send some samples to USA for mRNA seq. Since I can't prepare the cDNA here, I need to send the RNA. I thought to precipitate it and then send at 4°C. Anybody has never done this? Or anybody has alternative options? Moreover, someone knows which carrier is the best in mRNA precipitation (acrylamide or glycogen)?? Can they interfere with the following protocol? Many thanks Francesca

05-07-2013, 05:03 PM	#2
nkaushik Junior Member Location: UK Join Date: Mar 2012 Posts: 3	The best will be to send total RNA in 0.1M soduim acetate and 2 vol ethanol. I used to send total RNA to US for Affymetrix and Exiqon microarray experiments. Hope this helps Narendra

05-07-2013, 06:10 PM	#3
kcchan Senior Member Location: USA Join Date: Jul 2012 Posts: 185	If you have not already extracted your RNA, you can also lyse the samples in Trizol and ship the samples while frozen in Trizol. If the other lab is unable to perform a precipitation, you can also use RNAStable to store your sample. Your samples can then be shipped without dry ice and rehydration is as simple as adding water. The protocol is a bit more complicated for you, but it's much easier for the end user.

05-07-2013, 07:54 PM	#4
Francy87 Member Location: Italy Join Date: May 2013 Posts: 28	Hi! Which brand produce RNA stable? Do you know if it has been used for RNA for Illumina seq?

03-09-2015, 04:02 PM	#7
kclueneberg Junior Member Location: Mexico Join Date: Mar 2015 Posts: 1	Hello, Has anyone else have use RNAstable, what are your experiences with this product? Thanks Kind regards Kathia

03-10-2015, 05:10 AM	#8
MU Core Member Location: Columbia, Missouri Join Date: Apr 2008 Posts: 57	Our facility has used the RNA stabilization product from Gentegra with success. Samples are shipped at room temperature and remain stable. Upon arrival the RNA is resuspended and suitable for downstream applications such as an RNA-Seq library. http://www.gentegra.com/

03-10-2015, 07:49 AM	#10
MU Core Member Location: Columbia, Missouri Join Date: Apr 2008 Posts: 57	The cost through the US distributor is ~$4.00 per sample. There are 96-well plate options that reduce per sample cost below $2.00. http://www.gentegra.com/distributor.html