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Old 08-29-2013, 03:14 AM   #1
DavyK
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Location: Cardiff

Join Date: Jun 2012
Posts: 9
Default Ion torrent PCR duplicates

Hi All,

I am looking at some targeted ion torrent sequencing data. Over 90% of mapped reads are PCR duplicates. Is this normal? I thought it might be because the target is very small (11kb, 16 individuals), but I honestly am not sure.

Any ideas, given the target size how many duplicates I should be expecting?

Cheers,
Davy
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