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Old 09-02-2013, 07:50 PM   #1
Jimmy SHAO
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Location: Beichen West Road, Chaoyang District, Beijing, China

Join Date: Sep 2013
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Default Differential expression analysis about 80 genes

Recently, I been worked with RNA sequencing data analysis concerning a set of 80 candidate genes. Here is my library preparation workflow, RNA was reverse transcribed to cDNA which was used for capture by Angilent customized probes, then proceed to high through sequencing. After mapping, I want to do DEG analysis, which software should I to use when my gene number is limited? or I just calculate RPKM and simply identify DEGs by fold change > 2?
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